Simultaneous detection of HSV-1, HSV-2 and VZV in clinical samples by multiplex polymerase chain reaction (MPCR)
نویسندگان
چکیده
منابع مشابه
Detection of Ureaplasma Urealyticum in Clinical Samples from Infertile Women by Polymerase Chain Reaction
Genital Ureaplasma urealyticum infection is considered to be a sexually transmitted infection. The bacterium has been found to be involved in PID, chorioamnionitis, urethritis, respiratory distress syndrome and pneumonia in newborn, abortion and infertility. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. The purp...
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BACKGROUND & OBJECTIVES Polymerase chain reaction (PCR) has been known to be a rapid and accurate diagnostic test for causative viruses of viral retinitis, but cost is the limiting factor. In the present study an attempt was made to standardize a multiplex PCR (mPCR) on intraocular specimens from patients with viral retinitis for the detection of one or more viruses [herpes simplex virus (HSV),...
متن کاملDetection of Varicella Zoster Virus (VZV) in the Benign and Malignant Breast Tumors by Polymerase chain Reaction
This study aimed to investigate the frequency of Varicella Zoster Virus (VZV) in benign and malignant breast tumors. A total of 24 carcinomas and 24 fibroadenomas paraffin embedded tumoral tissue samples were obtained from the pathology sections of Toos and Firoozgar hospitals in Tehran, Iran. All samples were collected from patients from June 2011 to February 2012. DNA was extracted from all s...
متن کاملDevelopment of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses
Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...
متن کاملRAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN REACTION
We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...
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ژورنال
عنوان ژورنال: Journal of Clinical Virology
سال: 2006
ISSN: 1386-6532
DOI: 10.1016/s1386-6532(06)80792-4